2′-fucosyllactose (2′-FL)-CAD Detector test report

2′-fucosyllactose (2′-FL)(2′-FL) is an oligosaccharide that is abundant in breast milk and is a type of human milk oligosaccharide (HMOs). It has a variety of physiological functions, including promoting the growth of probiotics, inhibiting harmful bacteria, and immune regulation. it has been used by the u.s.FDA Approved as "Generally Recognized as Safe" (GRAS) and added as a food ingredient to infant formula.

in 2′-FL During the analysis and detection,high-performance liquid chromatography (HPLC)-Charged Aerosol Detector (CAD)(HPLC-CAD) plays an important role.HPLC-CAD Due to its high sensitivity and wide linear range, it is suitable for 2′-FL qualitative and quantitative analysis. For example, in the China Institute of Metrology jointly developed with a dairy company in Heilongjiang 2′-FL Among the purity standard materials, the HPLC-CAD Qualitative and quantitative analyzes were performed on the content of structurally relevant impurities.

In addition,HPLC-CAD Technology is also used to determine 2′-FL Characteristic values of purity reference materials, including purity 99.2%of 2′-FL The standard material and purity are 89.5%LNnT standard material. The establishment of these standard materials helps to ensure 2′-FL The accuracy and traceability of content determination are of great significance for the development of new formulas, product quality control and the establishment of absolute quantitative methods.

Charged Aerosol Detector (CAD)(charged aerosol detector,CAD) is a new general-purpose detector developed in recent years. Its detection has nothing to do with the molecular structure of the substance being measured, and the substance being measured does not need to be ionized. It can be used as long as the substance being measured is a semi-volatile or non-volatile compound.CAD detection. At the same time,CAD It is a mass-sensitive detector. The response value of the measured substance is determined by the absolute mass of the sample being tested. As long as the absolute mass of the sample being tested is consistent, the measured response value will basically be the same, and a lower detection limit and higher sensitivity can be obtained.

1, instruments and materials

Instruments:Agilent1200 liquid chromatograph+Charged Aerosol Detector (CAD)(INSTRUMAX, Model: CADetector a1)

Reagents: acetonitrile (chromatography grade), pure water (Watson's distilled water)

Sample: lactose, galactose,2′-fucosyllactose (2′-FL)

2, methods and results

2.1 CAD Conditions

Instrument model:INSTRUMAX CADetector a1

Drift tube temperature:40℃

Atomized air flow:3L/min

Polarization current:1μA

Polarized airflow:1L/min

2.2 Experimental results

3, experimental conclusion

HPLC-CAD As an efficient detection method, for 2′-FL of great value in research, development and quality control. With 2′-FL The application potential in the field of food nutrition is constantly being explored.HPLC-CAD Technology is expected to play an even more important role in this area.