The Charged Aerosol Detector (CAD) has become a highly versatile detection method in liquid chromatography due to its broad response capability to non-volatile compounds, excellent quantitative performance, and insensitivity to target structure.
In recent years, with the acceleration of the localization of high-end analytical instruments, the Charged Aerosol Detector independently developed by Instrumax has continuously achieved technological breakthroughs in key performance aspects such as sensitivity, stability, and consistency, gradually realizing substitution for similar foreign products. Hydrocortisone, as a typical steroid drug, lacks strong UV-absorbing groups in its molecule, posing certain challenges for detection in complex matrices.
Due to its stable structure, moderate solubility, and wide application in clinical drugs and raw material quality control, hydrocortisone is an ideal model compound for evaluating CAD detection performance. This report uses hydrocortisone as a representative sample to conduct systematic performance evaluation based on Instrumax CAD, covering indicators such as method establishment, concentration-response relationship, repeatability, baseline noise level, and minimum detectable amount. It aims to verify the instrument's applicability and stability in steroid compound analysis and provide technical support for its application in drug development, quality control, and regulatory analysis scenarios. Click to view full text