mono-tert-butyl eicosanedioate-CAD Detector test report

mono-tert-butyl eicosanedioate(CAS：683239-16-9) is an indivisible ADC Connectors for the synthesis of antibody-drug conjugates (ADC),mono-tert-butyl eicosanedioate Also an alkyl chain based PROTAC Connecting agent, can be used to synthesize PROTAC.

mono-tert-butyl eicosanedioate(20-(tert-Butoxy)-20-oxoicosanoic acid) is a long-chain alkyl diacid derivative, which is acidic and can be synthesized through esterification reaction. It is mainly used as an intermediate in organic synthesis. This compound is of great significance in the field of drug synthesis, especially in the synthesis of biologically active molecules.For example, it is used to prepare bioactive molecule insulin derivatives for the treatment of diseases such as diabetes and obesity.

in preparation mono-tert-butyl eicosanedioate During the process, due to the weak UV absorption of the target object and its related substances,Charged Aerosol Detector (CAD) It can be used as an efficient detection method to monitor the reaction process and identify the purity of the product.HPLC-CAD It is used to detect intermediates and final products during the synthesis process, ensuring high selectivity and high yield of the synthetic route.Charged Aerosol Detector (CAD) Played a key role in ensuring the efficiency of the synthetic route and quality control of the products.

1, instruments and materials

Instrument: Hitachi Primaide PLUS liquid chromatograph

Charged Aerosol Detector (CAD)(INSTRUMAX, Model: CADetector a1)

Series detector detection, UV detector in front,Charged Aerosol Detector (CAD) After.

Reagents: pure water (Watson's distilled water), acetonitrile (chromatography grade), trifluoroacetic acid (analytical grade)

Sample:mono-tert-butyl eicosanedioate crude product(2 species)

2, methods and results

2.1 Chromatographic conditions

Column:C18 column 250*4.6mm,5μm

Column temperature:45℃

Flow rate:1mL/min

Injection volume:2μL and 20μL

Mobile phase A:0.1%TFA Aqueous solution Mobile phase B: acetonitrile

Gradient conditions:

UV detector conditions:214nm

Charged Aerosol Detector (CAD) Conditions:

Instrument model:INSTRUMAX CADetector a1

Drift tube temperature:40℃

Atomized air flow:3L/min

Polarization current:1μA

Polarized airflow:1L/min

Gain：0.05

2.2 Sample preparation

Thinner: Methanol

sample 1: Sample weighing approx.30mg, set 10mL Volumetric flask, dissolve with methanol and dilute to the mark, that is (sample concentration: approx.3mg/mL).

sample 2: Sample weighing approx.100mg, set 10mL Volumetric flask, dissolve with methanol and dilute to the mark, that is (sample concentration: approx.10mg/mL).

2.3 Experimental results

2.3.1 sample 1

Result: Concentration 3mg/mL, injection 2μL;

Charged Aerosol Detector (CAD), the main peak is not flat, and the impurity detection is normal;

The main peak of the UV detector is small and impurities cannot be detected

Figure 1: sample 1-CAD Detector and UV detector test results

Figure 2: sample 1-CAD Magnified view of part of the detector

2.3.1 sample 2

Result: Concentration 10mg/mL, injection 20μL;

Charged Aerosol Detector (CAD), the main peak has a flat head, and the impurity detection is normal;

The main peak of the UV detector is small and impurities cannot be detected

Figure 3: sample 2-CAD Detector and UV detector test results

Figure 4: sample 2-CAD Magnified view of part of the detector

3, experimental conclusion

It can be seen from the experimental results:

Charged Aerosol Detector (CAD) Yes mono-tert-butyl eicosanedioate It has good response to its related substances and has greater detection advantages compared with UV detectors.

4, discuss

Charged Aerosol Detector (CAD) in mono-tert-butyl eicosanedioate can play an important role in the synthesis process:

1. Monitor reaction progress:Pass HPLC-CAD This method can monitor the intermediates and final products in the synthesis process in real time, understand the progress of the reaction in time, and adjust the reaction conditions.
2. Improve purity:Charged Aerosol Detector (CAD) It has high sensitivity and can effectively detect trace impurities, helping researchers evaluate the purity of the synthetic route and ensure the quality of the final product.
3. Optimize yield: During the synthesis process, by Charged Aerosol Detector (CAD) Quantitative analysis of intermediates and products in each step can optimize reaction conditions and increase the yield of target compounds.
4. Selectivity analysis:Charged Aerosol Detector (CAD) The response to a compound is independent of its structure, which facilitates selective analysis of specific compounds during synthesis and avoids unnecessary side reactions.
5. Industrialization amplification:Due to Charged Aerosol Detector (CAD) With its high sensitivity and stability, it is suitable for online monitoring in industrial production and helps to achieve scale and automation of the synthesis process.
6. Data recording and analysis:Charged Aerosol Detector (CAD) It can provide accurate chromatograms, provide data support for the recording and analysis of the synthesis process, and help researchers optimize and improve methods.
7. Environmentally friendly: Compared with traditional detection methods, using Charged Aerosol Detector (CAD) No need to use harmful derivatization reagents, more environmentally friendly.

To sum up,Charged Aerosol Detector (CAD) in mono-tert-butyl eicosanedioate During the synthesis process, it provides important support for the optimization of the synthesis process by monitoring the reaction progress, improving product purity, optimizing yield, analyzing selectivity, supporting industrial amplification, providing data recording and analysis, and being environmentally friendly.