CAD Applicationcough syrup Ingredient analysis
Eyes that explore the unknown world
1. Application background introduction
Experimental background and conditions
Determination of specific sugar components (fructose,glucose, sucrose)
Provides deeper information to achieve refined quality control and
Scientific production process, transparent product labeling, and anti-counterfeiting and authenticity
An indispensable technical foundation for precision.
CAD With high sensitivity, good repeatability and good linearity, it is ideal for the detection of carbohydrate components.
An effective tool for testing.
Experiment purpose
HPLC-CAD Detection of specific sugar components in syrups
Sample information
fructose,glucose, sucrose reference substance,cough syrup sample
Instrument information
Agilent 1200 liquid chromatograph+INSTRUMAX Charged Aerosol Detector (CAD)
（Instrumax CADetector a1）
2. Application content
Charged Aerosol Detector (CAD) Conditions
Experimental methods
Chromatography Column: Sugar Column 5μm 4.6*250mm
Mobile phase: acetonitrile: water (77:23)
Flow rate:1.0mL/min
CAD Conditions
Drift tube temperature: 35°C
Atomizing air flow: 3L/min
Charging airflow: 1L/min
Gain: 0.05
RF: 2.0
The three sugars are well separated, with sharp peak shapes, high column efficiency and high detection sensitivity.
Linear
The three sugar correlation coefficients R are all greater than 0.999
Injection precision
continuous 6 Needle, area of three sugar peaks RSD% are less than 1.0%
Sample testing
Double-sample double-needle content determination, accurate results and small deviation
3. Experimental conclusion
This study usesHPLC-CADDetermination of sugar in syrup
class component,CAD High sensitivity, good repeatability, linearity
Excellent, it is an effective tool for detecting sugar components. This experiment
Easy to operate, accurate and reliable results, for carbohydrate components
The determination provides powerful instrument and method support.