Charged Aerosol Detector (CAD) Application Brief--
D-allulose Detection

1, Preface

D-allulose(i.e.allulose) exists in small amounts in nature in figs and other natural foods, and its sweetness is about that of sucrose.70%, and after being ingested by the human body, most of them 6 Eliminated from the body within hours,Almost does not participate in human metabolism and has extremely low calories. Its sweetness is pure,The taste and volume characteristics are very similar to sucrose.. What's even better is that it is also a functional ingredient that is beneficial to human health.

Existing animal and human experiments show that D-allulose Can inhibit the small intestine glucose absorption, improve insulin sensitivity, therebyReduce blood sugar spikes; Able to regulate fat metabolism,Reduce lipid content in plasma and liver, reduces fat accumulation and is thought to haveFight obesitypotential; in addition,D-allulose It also has certain antioxidant and anti-inflammatory abilities.

The characteristics of "delicious + healthy" make allulose He has almost become an "international superstar" in the sugar substitute industry.

Charged Aerosol Detector (CAD)(charged aerosol detector,CAD) is a new general-purpose detector developed in recent years. Its detection has nothing to do with the molecular structure of the substance being measured, and the substance being measured does not need to be ionized. It can be used as long as the substance being measured is a semi-volatile or non-volatile compound.CAD detection. At the same time,CAD It is a mass-sensitive detector, the response value of the tested substance is determined by the quality of the sample being tested. As long as the quality of the sample being tested is consistent, the measured response value will basically be the same, and a lower detection limit and higher sensitivity can be obtained.

Charged Aerosol Detector (CAD) Yes INSTRUMAX The R&D team has achieved major scientific and technological achievements after years of painstaking research and repeated experiments. The detector uses advanced electricity and atomization technology to achieve high-sensitivity and high-precision detection of trace samples.

This article uses Charged Aerosol Detector (CAD), yes D-allulose Examine the detection methods,High sensitivity and good linearity, for D-allulose The detection provides a new highly sensitive detection method for better D-allulose Quality control provides new ideas.

2, instruments and materials

Instruments:Agilent1200 liquid chromatograph

Charged Aerosol Detector (CAD)(INSTRUMAX, Model: CADetector a1)

Reagents: pure water (Watson's distilled water),

Sample:D-allulose

3, methods and results

3.1 Chromatographic conditions

Mobile phase: pure water

Chromatographic column: Hydrogen sugar column,300mm✖7.8mm,8μm

Column temperature:80℃

Flow rate:0.6mL/min

CAD Conditions

Drift tube temperature:35℃

Atomized air flow:3L/min

Polarized airflow:1L/min

Polarization current:1μA

Gain：0.05

RF：2.0

3.2 Experimental results

3.2.1 Typical spectrum

20μg/mL sample, injection 10μL, the peak time is moderate, the peak shape is good, and the tailing factor is only 1.08, the number of theoretical plates reaches 17228.

3.2.2 Sensitivity

5μg/mL sample, injection 10μL, the signal-to-noise ratio reaches 16.70;With differential detector RI Compared to (RI The minimum detection concentration is 500μg/mL) The detection concentration is greatly reduced and the sensitivity is greatly improved.

3.2.3 Linear

5μg/mL,20μg/mL,50μg/mL,100μg/mL,200μg/mL,500μg/mL Each sample is injected 10μL, use sample concentration and peak area to make a linear equation.

Good linearity,D-allulose The correlation coefficient R is 0.99988, good linearity.

4, conclusion

This study uses HPLC-CAD Legal testing D-allulose, good peak shape, high sensitivity and good linearity; use CAD Easy to operate, accurate and reliable results, for D-allulose It provides strong instrument and method support for the determination of its related products.