Charged Aerosol Detector (CAD) Application Brief--
Nutritional Enhancers-D-ribose Detection

1, Preface

D-ribose It is a naturally occurring aldopentose (five-carbon sugar) with the chemical formula C₅H₁₀O₅. It is an important functional sugar in the body and is widely involved in energy metabolism and genetic information transmission.

Recently, the National Health Commission officially issued an announcement approving D-ribose As a new type of food nutrition fortifier, it is suitable for sports nutrition food (daily addition amount 1-2g), and included in the GB2760-2024 Catalog of synthetic flavors for food. This policy breakthrough provides a new direction for sports and health food innovation, and also brings more scientific functional choices to consumers!

Charged Aerosol Detector (CAD)(charged aerosol detector,CAD) is a new general-purpose detector developed in recent years. Its detection has nothing to do with the molecular structure of the substance being measured, and the substance being measured does not need to be ionized. It can be used as long as the substance being measured is a semi-volatile or non-volatile compound.CAD detection. At the same time,CAD It is a mass-sensitive detector. The response value of the substance being measured is determined by the quality of the sample being tested. As long as the quality of the sample being tested is consistent, the measured response value will basically be the same, and a lower detection limit and higher sensitivity can be obtained.

Charged Aerosol Detector (CAD) yes INSTRUMAX The R&D team has achieved major scientific and technological achievements after years of painstaking research and repeated experiments. The detector uses advanced electricity and atomization technology to achieve high-sensitivity and high-precision detection of trace samples.

This article uses Charged Aerosol Detector (CAD), yes D-ribose The detection method was investigated and found to have high sensitivity, good repeatability and good linearity.D-ribose The detection provides a new highly sensitive detection method for better D-ribose Quality control provides new ideas.

2, instruments and materials

Instruments:Agilent1200 liquid chromatograph

Charged Aerosol Detector (CAD)(INSTRUMAX, Model: CADetector a1)

Reagents: pure water (Watson's distilled water),

Sample:D-ribose

3, methods and results

3.1 Chromatographic conditions

Mobile phase: pure water

Chromatographic column: Hydrogen sugar column,300mm✖7.8mm,8μm

Column temperature:60℃

Flow rate:1mL/min

CAD Conditions

Drift tube temperature:40℃

Atomized air flow:3L/min

Polarized airflow:1L/min

Polarization current:1μA

Gain：0.05

3.2 Experimental results

3.2.1 20μg/mL sample, injection 10μL, the signal-to-noise ratio reaches 437;with RI Compared to (RI The detection concentration is 20mg/mL) The detection concentration is greatly reduced and the sensitivity is greatly improved.

3.2.2 Injection precision,200μg/mL sample, injection 10μL, continuous injection 6 needle, peak area RSD% is 1.198%(<2.0%), good repeatability

3.2.3 Linear

Inject 1μL,2μL,5μL,10μL and 20μL, use injection volume and peak area to make a linear equation.

Good linearity,D-ribose The correlation coefficient R is 0.99991, good linearity.

4, conclusion

This study uses HPLC-CAD Legal testing D-ribose, high sensitivity, good repeatability and good linearity; use CAD Easy to operate, accurate and reliable results, for D-ribose It provides strong instrument and method support for the determination of its related products.